Lymphatic Metabolism
This study investigates the mitochondrial bioenergetics of collecting lymphatic vessels in both healthy and disease states. The lymphatic system plays a crucial role in fluid homeostasis, immune cell trafficking, and lipid absorption, and its dysfunction is associated with pathological conditions such as lymphedema and lymphatic-related disorders. Mitochondria are essential for cellular energy production and signaling, yet their role in the function and dysfunction of collecting lymphatic vessels remains poorly understood.
The project aims to characterize mitochondrial respiration, ATP production, oxidative stress, and metabolic flexibility in lymphatic endothelial and lymphatic muscle cells under physiological and pathological conditions. By utilizing high-resolution respirometry, fluorescence-based metabolic assays, molecular techniques and ex-vivo and in-vivo lymphatic vessels contractility analysis we assess how mitochondrial dysfunction contributes to impaired lymphatic contractility, flow regulation, and overall lymphatic system function. Furthermore, we will explore potential therapeutic interventions targeting mitochondrial pathways to restore lymphatic bioenergetics and contractility and improve lymphedema and other lymphatic-related disorders outcomes.
Contact: Zhanna Nepiyushchikh